First pregnancies in jennies with vitrified donkey semen using a new warming method

Sperm vitrification has been recently developed, but fertility trials have not performed yet in equine species. In this study, a new warming technique for vitrified donkey semen was developed and the uterine inflammatory response were compared to conventional freezing. Experiment 1, sperm straws warmed 3 ml of extender or water bath at: 37 °C/30 s; 43 °C/10 60 °C/5 s. motility, plasma acrosome membranes DNA integrity between treatments. 2, jennies inseminated twice (500 × 106 sperm) body either with frozen (2 cycles/jenny). Pregnancy rates (polymorphonuclear neutrophil concentration; PMN) evaluated after artificial insemination (AI). No differences extender/water found s better than lower temperatures terms total (53.8 ± 13.2%) progressive motility (41.4 11.4%). PMN concentration (× 103 PMN/ml) (276.8 171.6) (309.7 250.7) AI. However, decreased faster (P < 0.05) using semen. greater (22%) (10%) statistically different. conclusion, could be directly at s, reducing obtained AI promoting positive pregnancy outcomes. These findings confirm possibility use as an alternative jennies.